Detection of influenza C virus by using an in situ esterase assay
نویسندگان
چکیده
منابع مشابه
A Sensitive Neutralization Assay for Influenza C Viruses Based on the Acetylesterase Activity HEF Glycoprotein
Influenza C virus possesses specific neuraminate-O-acetylesterase as a receptor-destroying function. This enzymatic activity of the viral glycoprotein HEF (Hemagglutinin, esterase activity and fusion factor) can be visualized in situ by the use of distinct color substrates. Hereby the localization, as well as the quantity of synthesized HEF protein is detectable. We further developed the estera...
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Detection of influenza C virus by a real‐time RT‐PCR assay
BACKGROUND Influenza C virus can cause both upper and lower respiratory tract infections and has been reported to be prevalent in children. However, these infections have been under-diagnosed, and epidemiological data available are limited due to the lack of convenient detection assays. OBJECTIVE Design and validate a real-time reverse-transcriptase PCR (rt RT-PCR) assay for the detection of ...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1989
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.27.5.832-836.1989